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Thermo Fisher Scientific Ion GeneStudio S5 DNA Sequencer


Ion GeneStudio S5 designed to offer high-quality sequencing results for a range of applications, including cancer research, microbial genomics, and genetic disease studies.

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Ion GeneStudio S5 is a next-generation sequencing (NGS) platform developed by Thermo Fisher Scientific. It is designed to offer high-quality sequencing results for a range of applications, including cancer research, microbial genomics, and genetic disease studies.

The Ion GeneStudio S5 system uses semiconductor sequencing technology, which is based on the detection of hydrogen ions released during nucleotide incorporation. This approach enables fast and accurate sequencing, with a typical run time of just a few hours.

The system is also highly flexible, with a range of different chips and sequencing kits available to accommodate different sample types and research goals. Additionally, the Ion GeneStudio S5 platform includes software tools for data analysis and interpretation, making it a comprehensive solution for NGS research.

Next-generation sequencing (NGS) is a high-throughput methodology that enables rapid sequencing of the base pairs in DNA or RNA samples. Supporting a broad range of applications, including gene expression profiling, chromosome counting, detection of epigenetic changes, and molecular analysis, NGS is driving discovery and enabling the future of personalized medicine.

Ion GeneStudio S5 Key Features:

  • Simplified software, with interactive touch screen for a better interface with the user.
  • Connectivity: wired Ethernet connection.
  • Remote monitoring capability.
  • Internet connection and Thermo Fisher Cloud account required.


Five different Ion S5TM chips optimize performance from 2M to 130M reads per run

Sequencing and analysis in just 3 hours with the Ion GeneStudio S5 Prime

All necessary automation:

  • Creation of libraries and preparation of the chip

30 minutes of labor to create libraries, tamper and load the chip using the Ion Chef system

  • Sequencing

2.5 hours – complete Ion GeneStudio S5 sequencing cycle, process preparation takes less than 15 minutes

  • Data analysis

Turn data into discoveries – analyze, track quality, and search for options using Thermo Fisher™ cloud services or your personal Ion Reporter™ server


  • cancer research
  • Hereditary Disease Research
  • Gene Expression Analysis
  • Reproductive Health Research
  • Microbiology/Infectious Disease Research ​

The Ion GeneStudio S5 Series

The Ion GeneStudio S5 Series sequencers share the same foundational technology and fluidic architecture across all three instruments: this Ion GeneStudio S5 System, the Ion GeneStudio S5 Plus System, and Ion GeneStudio S5 Prime System. The primary difference across the three instruments is on-sequencer analysis time.


The Ion GeneStudio S5 Series leverages the speed of semiconductor sequencing to enable the production of high quality sequencing data in a few hours and enables you to go from DNA library to data in as little as 24 hours with only 45 minutes of total hands-on time when paired with the Ion Chef System.


With single-day installation, a straightforward user interface, and cartridge-based reagents, the Ion GeneStudio S5 Series sequencers are easy to use. Combined with Ion AmpliSeq technology for target selection, the Ion Chef System for automated library and template preparation, and Ion Reporter Software for automated variant annotation, targeted sequencing is simpler than ever.

Small sample input

Low input DNA from challenging samples types such as formalin-fixed paraffin-embedded (FFPE) tissue, retrospective samples from fine needle aspirates, and cell-free DNA extracted from blood (cfDNA) can be difficult to sequence on competitor next-generation sequencers. However, with Ion AmpliSeq technology and the Ion GeneStudio S5 Series, you can use as little as 1 ng input DNA or RNA.


Why buy multiple systems for your lab when you can run all of your targeted sequencing or microbial applications on a single next-generation sequencer? The Ion 5 Series chips that run on the Ion GeneStudio S5 Series provide several throughput options, so your lab has the flexibility to scale from small to large projects, enabling multiple targeted sequencing applications on a single system.

Simple analysis and storage solutions

Torrent Suite Software and Ion Reporter Software make NGS easy for beginners and experts alike. Plan, monitor, track, and analyze your runs using Torrent Suite Software (pre-installed on the Torrent Server). Integrate, annotate, and interpret variants using Ion Reporter Software (Thermo Fisher Cloud or local options available based on your needs).

Services and support

With more than 2,000 field personnel, Thermo Fisher Scientific has the most extensive network of dedicated Field Application Specialists and Service Engineers to work with you, so you can be confident that we will be there when and where you need us most.


  • Electrical Requirement: 100–240 VAC, 50/60 Hz,6.5–14.5 A.
  • Environmental requirements:
  • Temperature: 20–30°C (68–86°F)
  • Humidity: 40–60%
  • Altitude: 2,000m (6,500ft)
  • Power: 1,200W
  • Compatible Chips: Ion 510, 520, 530,and 540 Chips
  • Storage space Server:  12 TB
  • Connection type:  1 GigE Ethernet; 2 x USB 2.0; RJ45-type connector
  • Dimensions : (Depth x Width x Height) 54.2 x 80.6 x 50.9 cm
  • Weight : 63.5kg


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