Lonza 4D Nucleofector System Core Unit With X Unit

Description

The Lonza 4D-Nucleofector is a specialized instrument used for transfection, a process of introducing foreign genetic material into cells. It is designed to deliver nucleic acids, such as plasmid DNA or RNA, into a wide range of cell types with high efficiency.

The 4D-Nucleofector system combines electrical and mechanical forces to facilitate the uptake of nucleic acids by cells. It uses a proprietary electroporation technology that generates an electric field to create temporary pores in the cell membrane, allowing the nucleic acids to enter the cells. This process is often referred to as electroporation-based transfection.

Lonza 4D-Nucleofector FEATURES:

• Controls attached functional units
• 5.7″ foldable touch screen to operate the system
• Intuitive operation software for designing and saving individual experimental setups
• USB port for software update and data transfer
• Includes USB and serial connectivity for the 96-well Shuttle Add-on

The “4D” in the name of the instrument refers to the four dimensions of transfection that the system addresses:

• Optimization: The system allows for optimization of transfection conditions, including electric parameters, for different cell types and applications.
• Standardization: It provides standardized protocols to ensure consistent and reproducible results across experiments.
• Flexibility: The system supports a wide range of cell types, including primary cells, stem cells, and hard-to-transfect cells.
• Scalability: It allows for both small- and large-scale transfections, accommodating various research needs.

The Lonza 4D-Nucleofector is commonly used in molecular biology and biotechnology research laboratories for applications such as gene expression studies, protein production, genome editing using CRISPR/Cas9, and functional genomics. By enabling efficient transfection, the instrument plays a crucial role in introducing genetic material into cells for manipulating their behavior and studying gene function.

What is the x unit means in Lonza 4D-Nucleofector

The term “x unit” in the context of the Lonza 4D-Nucleofector refers to a relative measure used to quantify the level of transfection efficiency achieved with the system. It is a unit of measurement specific to the 4D-Nucleofector and is used to compare the transfection performance of different cell types or experimental conditions.

The exact definition of an “x unit” can vary depending on the specific application and the experimental setup. Generally, it represents a relative measure of the amount of genetic material successfully delivered into cells. The higher the number of x units, the greater the transfection efficiency.

The specific calibration and determination of x units are typically established by Lonza, the manufacturer of the 4D-Nucleofector system, based on their internal testing and optimization for different cell types. Lonza provides guidelines and protocols to help researchers interpret the transfection efficiency in terms of x units and compare their results with reference values.

It’s important to note that the x unit is not a standardized or absolute unit of measurement used universally across different transfection technologies or instruments. It is specific to the Lonza 4D-Nucleofector system and serves as a relative measure within that particular context.

The Core Unit – Controlling the 4D-Nucleofector™ System

• 5.7’’ foldable touch screen to operate the system and controlling the functional units
• Intuitive operation software for designing and saving individual experimental setups
• USB port for software update and data transfer

The X unit – Supporting Nucleofection™ of various cell numbers in different formats

• Features positions for 20µl Nucleocuvette™ strips and 100 μl single Nucleocuvette™
• Seamless transfer of conditions between different Nucleofection™ vessels
• Comprises HV connectivity for the 96-well Shuttle™ Device

The Y unit – Enabling adherent Nucleofection™ in 24-well culture plates

• Nucleofection™ of neurons in adherence in any developmental stage
• Up to 70% efficiency and maintenance of functionality
• Suitable for confocal microscopy read-out