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Roche LightCycler 96 Real Time PCR System


LightCycler 96 Real-Time PCR system finds its application in detecting the coronavirus using the SARS-CoV-2 PCR detection.

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Roche LightCycler 96 Category:


LightCycler 96 Instrument is a real-time PCR system for rapid cycling up to 96 samples. It is an optical detection system that allows flexible detection of sequence-dependent probes, such as hydrolysis probes and sequence-independent dyes (i.e., SYBR Green I). Multiplex and multicolor capabilities permit the use of up to four different fluorescent dyes.

LightCycler 96 real-time PCR system from Roche (Germany) allows you to perform all major types of real-time PCR analysis, including the qualitative and quantitative determination of target nucleic acid sequences, genotyping with TaqMan probes, gene expression analysis, and HRM analysis already in the basic configuration of the device.

LightCycler 96 Real-Time PCR System – A Brief

PCR systems find prevalent usage in research labs nowadays. Be it their performance, size, or anything related to the machine, it intrigues the researchers to study more through them. If you are looking for a medium-throughout and lightweight system, the LightCycler 96 Real-Time PCR System is a perfect choice. Read to know more about this PCR system from Roche and its benefits. 

The LightCycler 96 module for high-resolution melting analysis will be suitable for human genetic disease research applications. It enables easy and fast detection of known and unknown gene mutations, including single-nucleotide polymorphisms, deletions and methylated regions.

LightCycler 96 Specifications: 

  • Measurement – 40 x 40 x 53 cm 
  • Temperature Range – 37-98 degrees 
  • Detection – Cooled CCD Camera 
  • Sensitivity – one copy 
  • Wells – 96 
  • Purpose – COVID-19 Diagnosis 

LightCycler 96 Features: 

The LightCycler 96 Real-Time PCR system finds its application in detecting the coronavirus using the SARS-CoV-2 PCR detection. The machine achieves the intended results with the help of the following features: 

  1. It comes with a plate-based system with 96 unique wells. 
  2. Its high-performance temperature gradient is efficient for assay optimization. 
  3. It provides a 4-plex multicolor operation. 
  4. This machine has one copy sensitivity and a dynamic range of 10 logs. 
  5. It is a standalone system and is integrable with a personal computer. 
  6. A separate instrument software with a touchscreen mechanism. It is free of cost and can loan on any PC. 
  7. It has a runtime of less than an hour. 
  8. It can perform relative and absolute quantification, endpoint genotyping, and melting curve analysis. 
  9. This PCR machine uses intuitive and state-of-the-art software for phenomenal results and a convenient interface. 
  10. Its PC analysis software is also free of charge and can be easily loaded on any personal computer. 

LightCycler 96 Advantages: 

As mentioned above, the main purpose of the LightCycler 96 Real-Time PCR system is COVID-19 detection. It eases the detection process and provides efficient results each time. The following are some of the many advantages of this PCR system. 

  1. This machine combines sturdy glass fibers with higher intensity and broad-spectrum LEDs to maximize the device sensitivity. It can obtain the detection of all the wells simultaneously in less than a second. 
  2. It provides free-to-use software that fits according to the operator’s needs like the following: 
  3. As a standalone machine. We can use a flash drive to transfer the data. 
  4. We can directly connect the machine to a PC. 
  5. We can connect the machine to any PC using a LAN network or Ethernet connectivity. 
  6. Its state-of-the-art analysis methods help in extracting only useful and meaningful data. These in turn export the obtained results in an RDML format (ready to publish). 
  7. Its cutting-edge low-mass thermal block design accounts for the machine’s homogeneity. Also, they provide broad temperature ranges and accessible gradient features. 
  8. This machine uses an electroforming production process to fit the 96-well plate exactly to the block, which is a perfect balance between the positive (block) and negative (plate). This method also avoids air cushions that can potentially disturb the readings. 
  9. Its specific Application and Instrument Software provides an easy analysis workflow. It provides convenient import and export functions, email notifications after every run, a server-based network interface, and flexible online monitoring. 

Main characteristics

ControlBuilt-in intuitive touchscreen / PC control
Excitation channels, nm470, 533, 577, 645
Detection channels, nm514, 572, 620, 697
Recommended dyesFluorescent labeled probes: FAM, HEX, VIC, Joe, Yellow555, ROX, Red610, TexasRed, Cy5
Device dimensions (WxDxH, weight)40cm × 40cm × 53cm; 25 Kg
Expendable materialsStandard low-profile: – 96-well plates with or without a “ half- skirt” – stripped tubes of 8 pcs.
The volume of the reaction mixture10-50 μl
Accuracy of temperature maintenance± 0.2 ° C
Temperature homogeneity± 0.3 ° C
Temperature range37–98 ° C
Heating rate4.4 ° C / sec
Cooling rate2.2 ° C / sec


  • High Pure Viral RNA Kit – 100 purifications
  • Lightmix Modular SARS-CoV (COVID-19) TIB MOLBIOL
  • White stripped tubes for real-time PCR, with caps / LightCycler 8-Tube Strips (white), 120 strips / pack
  • 96-well clear plate (LightCycler480 Multiwell Plate 96, clear)
  • 96-well plate (LightCycler 480 Multiwell Plate 96)
  • Greiner Bio-One Roche LightCycler Compatible 96-Well Microplates
  • LightCycler RNA Amplification Kit HybProbe – 1 kit (for 96 reactions of 20 uL final reaction volume)
  • LightCycler RNA Master SYBR Green I – 1 kit (for 96 reactions of 20 uL final reaction volume)
  • High Pure RNA Isolation Kit
  • 2 lightcycler 96 quick guides: -system installation -programming and running an experiment 1 lightcycler 96 usb drive containing: -executable for the software -release notes -license texts -lightcycler 96 system guides decontamination and shipment preparation -demo data and experiment templates provided by roche
  • 1 mains power cable (eu)
  • 1 mains power cable (us)
  • 1 lan cable (3 m)
  • 2 ventilation dust filters
  • 1 fuse package fuse 5×20 t8ah 250v ulr/iec
  • 1 sealing foil applicator

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