Thermo Scientific Invitrogen Qubit 4 Fluorometer with WiFi

Description

The Qubit 4 Fluorometer, developed by Thermo Fisher Scientific, stands as a pivotal instrument in the domain of molecular biology and biochemistry. It provides a more precise and sensitive means of quantifying DNA, RNA, and protein in samples compared to traditional spectrophotometric methods. Analyzing the Qubit 4 Fluorometer from a research-focused viewpoint provides a comprehensive understanding of its capabilities and importance.

The Qubit 4 Fluorometer with WiFi is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays.

The Qubit 4 Fluorometer was re-engineered to enable data transfer via WiFi as well as to run the Qubit RNA IQ (integrity & quality) assay. The Qubit 4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps. For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Qubit 4 Key Features:

• High Sensitivity: Qubit 4 Fluorometer excels in detecting low concentrations of nucleic acids or proteins, making it essential for experiments that require precise quantification.
• Specificity: Unlike UV spectrophotometry, which can be influenced by any absorbing impurity, Qubit assays are designed to be highly specific to the molecule of interest, be it DNA, RNA, or protein.
• Broad Range: The device can measure samples over a wide concentration range, minimizing the need for dilutions.
• Intuitive Interface: The Qubit 4 offers a user-friendly touchscreen interface, simplifying workflow and enabling data storage and transfer.
• Assay Versatility: A variety of Qubit assays are available, allowing researchers to tailor their measurements to specific needs, such as dsDNA, ssDNA, RNA, or protein.

Research Implications:

• Sample Purity: As the Qubit 4 Fluorometer can differentiate between nucleic acids and contaminants, it’s invaluable in experiments requiring pure DNA or RNA, such as next-generation sequencing or qPCR.
• Proteomics: For protein researchers, accurate quantification is paramount, especially when studying protein-protein interactions or conducting enzymatic assays.
• Genomics and Transcriptomics: In genomics and transcriptomics research, accurate quantification of nucleic acids is pivotal for downstream applications.
• Validation Studies: The Qubit 4 can serve as a gold standard to validate findings from other quantification methods.

Significance in the Scientific Community:

Offering precision, specificity, and versatility, the Qubit 4 Fluorometer reshapes the standards for nucleic acid and protein quantification. Its role is indispensable in ensuring the accuracy and reliability of countless molecular biology experiments.

Qubit 4 Specifications:

Manufacturer: Thermo Scientific Model: Qubit 4 Cat. number: Q33226 Dynamic range: 5 orders of magnitude Processing time: ≤5 seconds/sample Light sources: Blue LED (max ~470 nm) Red LED (max ~635 nm) Excitation filters: Blue 430–495 nm Red 600–645 nm Emission filters: Green 510–580 nm Red 665–720 nm Detectors: Photodiodes with measurement capability from 300–1000 nm Calibration type: 2- or 3-point standard Tube type: 0.5-mL Real Time PCR (polypropylene) tubes Warm-up time: <35 seconds USB drive capacity: 4 Gigabyte Operating temperature: 10–40°C Operating humidity: 20–80% (non-condensing) Operating power: 100–240 VAC, 1.0 A/1.2 A Frequency: 50/60 Hz Electrical input: 12 VDC, 2.5 A Dimensions (WxDxH): 136 x 250 x 55 mm Weight: approximately 750 g

Including certificate of conformance, international power adapter, USB drive, USB cable and microfiber cloth

Conclusion:

The Qubit 4 Fluorometer stands as a beacon of innovation in molecular biology research. Developed by the renowned Thermo Fisher Scientific, it has set an unparalleled standard for the accurate and sensitive quantification of DNA, RNA, and proteins. Its high specificity, broad range, and versatile assay compatibility make it indispensable for ensuring research precision. For any lab aiming to stay at the forefront of molecular research, integrating the Qubit 4 Fluorometer into their toolkit is a strategic move, ensuring data accuracy and bolstering scientific integrity.

Reference

Manufacturer Information