Description
QIAGEN Rotor-Gene Q is a thermal cycling system for real-time high-precision polymerase chain reaction (PCR), endpoint PCR, and high-resolution melt analysis (HRM). It is ideal for gene expression analysis, genotyping, an indication of infectious agents, and many other research areas.
The Rotor-Gene Q real-time PCR instrument combines many optimized design features to deliver outstanding performance and reliable results your research demands. The rotor system of the reaction unit allows for maintaining the specified temperature and optimal reaction conditions for effective amplification and detection. It also makes it possible to simultaneously study up to 100 objects and significantly reduce the analysis time. This system allows you to easily configure, optimize, and create a protocol for the required type of Analysis.
Rotor-Gene Q is a high-sensitivity and precision thermal cycler for real-time PCR. Thanks to its HRM (High-Resolution Melting) channel, it allows the detection of SNP class 4. Rotor-Gene Q is the only one that has a rotary system architecture, regulating the temperature through airflow, which makes it more precise and uniform in control.
QIAGEN Rotor-Gene Q Advantages
- Real-time data display.
- Rotary reaction module.
- Possibility of using replaceable rotors for 36 and 72 samples.
- Ability to work with rotary plates for 72 samples.
- The expandable capacity of the reaction blocks up to 100 samples.
- Possibly carrying out multiplex polymerase chain reaction with the case of independent detection of at least 5 (five) DNA targets.
- Possibility of quantitative determination of amplification products.
- Possibility of using universal 0.2 ml PCR tubes with flat and convex lids.
- Possibility of using tubes in strips with a volume of 0.1 ml.
- The volume of the reaction mixture is 10 – 50 ÎĽl.
- Heating rate – not less than 15 ° С / sec.
- Cooling rate – not less than 20 ° С / sec.
- Uniformity of temperatures from sample to sample – no more than ± 0.02 ° С.
- Resolution for temperature setting (minimum programmable change) – no more than ± 0.02 ° С.
- Temperature range – from 35 to 99 ° С.
- Temperature measurement accuracy – no more than ± 0.5 ° С.
- Excitation sources are high-energy light-emitting diodes.
- The detector is a photomultiplier tube.
- The time to obtain a fluorescence indicator from one sample is no more than 4 seconds.
- Â The kits of domestic manufacturers are adapted to work on Rotor-Gene Q devices.
- Unrivaled thermal and optical performance for real-time PCR with the rotary format.
- Unrivaled optical range covering ultraviolet and infrared wavelengths.
- Perform modern analyzes with user-friendly software and optimized QIAGEN kits.
- Minimal variation in thermal and optical performance from sample to sample.
- Easy maintenance and maximum convenience thanks to robust design.
Streamlined Analysis to drive your Research Forward.
The Gene Q rotor combines many optimized design features to provide outstanding performance and reliable results you require. The unique centrifuge design makes the Gene Q Rotor the most accurate and versatile real-time PCR machine on the market. The combination of instruments and reagents for real-time PCR provides a comprehensive analytical solution for pathogen detection.
Peculiarities
- The rotary reaction chamber has excellent thermal and optical performance;
- Wide wavelength range from UV to IR;
- High performance;
- Reliable design of the device, coupled with simple maintenance, provide the most comfortable working conditions;
- Convenient interface;
- Calibration procedures are not required during operation;
- The same optical path length from the fluorescence excitation source to the sample and from the sample to the fluorescence detector for all working wells.
Application
- Identification of pathogens of infectious diseases, simultaneous determination of several pathogens in one sample
- Genotyping of microorganisms
- Determination of viral load, monitoring the course of infection
- Assessment of gene expression level, Analysis of GMO concentration in food
- HRM analysis
Specifications
- Capacity: up to 100 samples per cycle.
- Temperature accuracy: ± 0.25 ° C (30 seconds after start).
- Temperature resolution: ± 0.02 ° C.
- Temperature uniformity: ± 0.01 ° C.
- Excitation source: high-energy photodiodes.
- Detector: photomultiplier tube.
- Capture time: 4 seconds.
- Power supply: 100-240 V, 50-60 Hz, 520 VA.
- Dimensions (WxDxH), mm – 370 mm Ă— 420 mm x 286 mm.
- Weight: 12.5 kg.
The Rotor-Gene Q Amplifier is a thermal cycling system for high-precision quantitative and qualitative real-time PCR analysis, PCR with endpoint detection. Together with the optimized QIAGEN kits, the instrument provides reproducible results for any experimental setup due to its high performance and robust design, as well as user-friendly software. The airflow offers the heating and cooling of the samples, which excludes the occurrence of the edge effect and guarantees the reliability of the results obtained.
Rotor-Gene Q Models:
- Rotor-Gene Q 6plex
Together with the optimized QIAGEN kits, the instrument provides reproducible results for any experimental setup due to its high performance and robust design, as well as user-friendly software. The airflow offers the heating and cooling of the samples, which excludes the occurrence of the edge effect and guarantees the reliability of the results obtained.
Rotor-Gene Q 5plex HRM
The Rotor-Gene Q 5 plex HRM Amplifier is a thermal cycling system for high-precision quantitative and qualitative real-time PCR (real-time PCR), endpoint PCR and high-resolution melt analysis (HRM). Together with Qiagen’s optimized kits, the instrument provides reproducible results for any experiment, thanks to its high performance and robust design, as well as user-friendly software. The airflow offers the heating and cooling of the samples, which excludes the occurrence of the edge effect and guarantees the reliability of the results obtained.
Rotor-Gene Q 5plex
The Rotor-Gene Q 5 plex Amplifier is a thermal cycling system for high-precision quantitative and qualitative real-time PCR, endpoint PCR, and high resolution melt Analysis (HRM). Together with Qiagen’s optimized kits, the instrument provides reproducible results for any experiment, thanks to its high performance and robust design, as well as user-friendly software. The airflow offers the heating and cooling of the samples, which excludes the occurrence of the edge effect and guarantees the reliability of the results obtained.
Rotor-Gene Q 2plex HRM
The Rotor-Gene Q 2 plex HRM Amplifier is a thermal cycling system for high-precision quantitative and qualitative real-time PCR (real-time PCR), endpoint detection, and high-resolution melt analysis (HRM). Together with Qiagen’s optimized kits, the instrument provides reproducible results for any experiment, thanks to its high performance and robust design, as well as user-friendly software. The airflow offers heating and cooling of the samples, which excludes the edge effect and guarantees the reliability of the results obtained.
Rotor-Gene Q 2plex
The Rotor-Gene Q 2 plex Amplifier is a thermal cycling system for high-precision quantitative and qualitative real-time PCR, endpoint PCR, and high-resolution melt analysis (HRM). Together with Qiagen’s optimized kits, the instrument provides reproducible results for any experiment, thanks to its high performance and robust design, as well as user-friendly software. The airflow offers the heating and cooling of the samples, which excludes the occurrence of the edge effect and guarantees the reliability of the results obtained.
The real-time PCR thermal cycler combines several optimized design features that provide the enormous performance and reliable results that your research needs. Together with QIAGEN’s optimized kits, the Rotor-Gene Q enables Analysis for a wide range of applications. It features new Q-Rex analysis software for life science qPCR applications, allowing you to add new functionality without affecting your work.
Rotary design
The unique rotary design of the Rotor-Gene makes it the most accurate and versatile qPCR Thermal Cycler available on the market. Each tube rotates in an air-heated chamber keeping all samples at the same temperature. In detection, each tube is aligned with the optics, the sample is illuminated, and the fluorescent signal is quickly captured from a single short optical path.
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