Agilent Cary Eclipse Fluorescence Spectrometer

Description

The Agilent Cary Eclipse is a fluorescence spectrophotometer. Fluorescence spectrophotometry is an analytical method used to measure the intensity of emitted light from a sample after exposure to excitation light. The Cary Eclipse is designed to provide high-performance fluorescence, phosphorescence, and chemi- and bioluminescence measurements.

The Cary Eclipse Spectrophotometer uses a Xenon flash lamp for superior sensitivity, high signal-to-noise, and fast kinetics. It measures the emission of light from samples in four modes. Using Xenon lamp technology, it captures a data point every 12.5 ms and scans at 24,000 nm/min without peak shifts. The Cary Eclipse is the only spectrophotometer with room light immunity. With an optional microplate reader, it offers full wavelength scanning of 384 samples.

Some notable features and advantages of the Agilent Cary Eclipse include:

• Sensitivity: The Cary Eclipse is known for its high sensitivity, which allows for detecting low concentrations of analytes in a sample.
• Versatility: The instrument can measure not only fluorescence but also phosphorescence, chemiluminescence, and bioluminescence, making it suitable for a wide range of applications.
• Fast Kinetics: The instrument has the capability to capture fast kinetic events, which is beneficial for certain advanced research applications.
• Temperature Control: Some models come with temperature control features crucial for studying temperature-dependent behaviors of certain compounds or reactions.
• Wide Wavelength Range: The Cary Eclipse covers a broad range of wavelengths, enabling the study of various fluorophores.
• Software: The instrument often comes bundled with sophisticated software that aids data collection, analysis, and interpretation.
• Multiple Sample Formats: Depending on the accessories, the Cary Eclipse can analyze samples in various formats, from liquid solutions in cuvettes to solid samples or thin films.

Applications for the Agilent Cary Eclipse might range from studying molecular interactions in biochemical research to quality control in manufacturing processes where fluorescence properties are of interest.

As with any instrument, while the Cary Eclipse offers a wide range of features and capabilities, it’s essential to ensure that the specific model and its accessories match the intended application’s requirements.

Refurbished system comprises

• Cary Eclipse
• Software Cary Eclipse Analysis Pack
• Cary Eclipse Country Kit – Europe
• 12 months Warranty

The Cary Eclipse Fluorescence Spectrophotometer is a product from Agilent Technologies, and its detector is an essential component that allows for the precise measurement of emitted light from a sample following its excitation.

Here’s a brief breakdown:

• Fluorescence Spectrophotometry Principle: When a sample absorbs light (photons) at a specific wavelength (excitation wavelength), it can emit light at a longer wavelength (emission wavelength). This emitted light is what’s measured in fluorescence spectroscopy.
• Detector’s Role: The detector’s primary function in the Cary Eclipse, or any fluorescence spectrophotometer for that matter, is to measure the intensity of this emitted light accurately.
• PMT (Photomultiplier Tube): Typically, fluorescence spectrophotometers, including the Cary Eclipse, use a photomultiplier tube (PMT) as the detector. PMTs are known for their high sensitivity, allowing for the detection of weak fluorescence signals. They work by amplifying the signal of received photons, converting them into a measurable electric current.
• Advantages: The Cary Eclipse detector is designed to offer high sensitivity and a broad dynamic range. This ensures that even samples with low fluorescence can be analyzed accurately. The system’s speed and precision are also beneficial for applications requiring rapid data acquisition or monitoring of fast kinetic events.
• Applications: With such a detector, the Cary Eclipse can handle various applications, from routine fluorescence measurements to advanced research in biochemistry, material science, and pharmaceuticals.

The detector, along with the instrument’s optics and other components, is pivotal in ensuring the accuracy, sensitivity, and repeatability of fluorescence measurements.

Specifications:

Wavelength range: 200 to 900nm Weight: 31 kg (68 lbs) Dimensions (W x D x H): 60 x 61 x 28 cm (24 x 24 x 11 in) Electrical: 100-240 VAC, 50/60 Hz

Includes:

• Cary Eclipse 96 & 384-Well BIO Microplate Reader
• Wavelength Range: 200 ~900 nm
• Microplate Reader Installedd
• System Control Computer(CPU only); that contains Program SW installed
• Operating Manual PDF
• PC Interface Communication Ready
• Interface & Connecting Cables
• Fully tested operationally.
• a PC monitor

Reference

• Manufacturer Information
• Brochure