Zeiss Axio Observer Z1 Inverted Microscope

Description

The Zeiss Axio Observer Z1 is a highly versatile inverted microscope system designed to meet the dynamic requirements of life sciences research. It provides interfaces for various technologies, from widefield transmitted light to sensitive super-resolution imaging and convenient 3D sectioning, making it suitable for complex imaging tasks. Researchers can choose the optimal incubation equipment for their experiments and benefit from easy sample access, which is essential for precise micromanipulation.

This system is particularly beneficial for materials science applications such as metallography. The inverted design of the Axio Observer allows for efficient investigation, development, and analysis of materials, especially metallographic specimens. Combining ZEISS’s renowned optics quality with automated components ensures reliability and reproducibility in research findings.

Features of the Carl Zeiss Axio Observer Z1 Inverted Microscope include:

• Optics for image generation
• High thermal and mechanical stability
• The high degree of flexibility for documenting results
• Improved ergonomics
• LCD for instrument parameters
• Instrument parameters are displayed on and can be configured using a TFT display
• 23 mm field of view
• Light Manager, Contrast Manager
• Modular design for optimum adaptation for different applications
• 6-position nosepiece
• 6-position reflector turret
• can be loaded in situ or removed for loading
• 5 or 6-position condenser turret
• 3-position Optovar turret
• Removable aperture stop and luminous-field stop sliders for reflected light
• Fluorescence shutter (internal standard shutter or external high-speed shutter)
• HAL 100, HBO 100, HBO 50 illuminators
• All essential microscope functions are motorized

The AxioObserver Z1 model stands out with its fully automated controls, including an objective nosepiece, reflector turret, condenser, and a fully motorized stage for x, y, and z focus control. It also features a sophisticated light path control system and Zen Blue 2.3 software, enhancing its capabilities for advanced research applications​.

Zeiss Axio Observer Z1 inverted microscope. The microscope is serviced and in good working condition. All optics are good, and all adjustments are working correctly.

Specifications:

Zeiss Axio Observer Z1 motorized

Opto-electronically encoded motorized XY stage 130*85 mm travel. Inserts for slides, 35 mm dishes and multiwell plates.

Pecon XL S1 incubator and control modules

X-CITE 120XL metal halide fluorescence light source

Filter cubes:

1. Analyzer
2. DAPI filter cube 49 (BP G365, FT 395, BP 445/50)
3. Cy5 filter cube (Semrock FF01-630/38, FF655-Di01, FF01-694/44)
4. GFP filter cube HE38 (BP 470/40, FT 495, BP 525/50)
5. YFP filter cube HE46 (BP 500/20, FT 515, BP 535/30)
6. Red filter cube HQ45 (BP 560/40, FT 585, BP 630/75)

FRET cube available (Semrock x438/24, FF458-Di01, m542/27) – swap into position 4 for ratiometric CFP/YFP FRET

Also available (please ask LMCF staff for them):

1. CFP filter cube HE47 (BP 436/20, FT 455, BP 480/40)
2. Cy7 filter cube (BP710/75, FT750, BP810/90)

Fluorescence attenuator:

• Open (100% Transmission)
• 70% T
• 50% T
• 40% T
• 20% T

• 2% T

Objectives:

• 2.5x/0.075 EC-Plan-NeoFluar (440310-9903), WD: 9.5mm
• 5x/0.16 Plan-NeoFluar (440320-9902), WD: 18.5mm
• 10x/0.30 Plan-NeoFluar Ph1 (440331-9902), WD: 5.2mm
• 20x/0.80 Plan-Apochromat DICII (440640-9903), WD: 0.55mm, Coverglass: 0.17mm
• 63x/1.4 Oil Plan Apochromat DICIII (440762-9904), WD: 0.19mm, Coverglass: 0.17mm
• 100x/1.46 (Oil) Plan Apochromat DICIII (420792-9800), WD: 0.11mm, Coverglass: 0.17mm, (UV)VIS-IR
• Available: 40x/1.3 Oil Plan-NeoFluar DICII (440452-9903), WD: 0.20mm, Coverglass: 0.17mm
• Available: 40x/0.75 EC-Plan-NeoFluar DICII (440350-9903), WD: 0.71mm, Coverglass: 0.17mm

Calibrations for Photometrics Evolve EMCCD and CoolSNAP ES2 cameras:

• 2.5x EMCCD px=6.1650 um, CoolSNAP px=2.420 um
• 5x EMCCD px= 3.0828 um, CoolSNAP px= 1.264 um
• 10x EMCCD px= 1.5556 um, CoolSNAP px= 0.613 um
• 20x EMCCD px= 0.7726 um, CoolSNAP px= 0.309 um
• 40x EMCCD px= 0.3810 um, CoolSNAP px= 0.157 um
• 63x EMCCD px= 0.2423 um, CoolSNAP px= 0.098 um
• 100x EMCCD px= 0.1558 um, CoolSNAP px=0.062 um

Cameras:

• Photometrics Evolve back-thinned EMCCD camera (512*512)
• Photometrics CoolSNAP HQ2 CCD camera (1392*1040)

Computer:

• HP Z4 G4 Workstation, Intel W-2133 3.6GHz processor, 32GB RAM, Nvidia Quadro P620 2GB graphics
• Windows 10 64-bit
• MetaMorph 7.10.1, system ID 32330
• ZEN Pro 2.3

Include:

• Zeiss Power Supply 232
• Zeiss 425537 binocular tube 45⁰/23
• Zeiss 455043-0000 W-Pl 10x/23 eyepiece set
• Zeiss 6 Place Nosepiece, H DIC Motorized
• Zeiss 420140-9900 Fluar 10x/0.50 ∞/0.17
• Zeiss 421360-9970 LD Plan-Neofluar 40x/0.6 Korr ∞/0-1.5
• Zeiss 421380-9970 LD Plan-Neofluar 63x/0.75 Korr ∞/0-1.5
• Zeiss 424947 6 position fluorescence reflector turret motorized with four fluorescence cubes, DAPI, FITC, TR. CY3
• Zeiss 424244 6 Place Motorized LD NA 0.55 WD 26mm turret condenser with Ph1, Ph2, and Ph3 annuli with open space to add DIC prisms.
• Zeiss 1x C-Mount on the left side. The microscope has an additional camera port on the right side.
• Zeiss HAL100 100W Halogen Lamp for transmitted light illumination.
• Zeiss HBO 100 Lamp with Power Supply for fluorescence.

The Zeiss Axio Observer Z1 is in excellent condition, clean, tested, and working. All optics are in perfect condition.